Journal: Biochemistry and Biophysics Reports

Article Title: TMEM207-mediated the impairment of skin regeneration through YAP sequestration in an allergic contact dermatitis model

doi: 10.1016/j.bbrep.2025.102409

Figure Lengend Snippet: (A): (left) Representative immunohistochemical staining of the skin using an anti -ABCB1 antibody. (center) Comparison of PPARg and SCD1 gene expression. (right) Representative immunohistochemical staining of the skin using an anti -SCD1 antibody. ∗∗ no significant difference; ∗P < 0.05. (B): Representative immunohistochemical staining of the skin using an anti -ki67 antibody and Nuclear Ki67 IHC score. Expression of ki67 was significantly reduced in Tg compared to wild type. ∗P < 0.05. (C): Representative immunohistochemical staining of the skin using an anti -Loricrin antibody. (D): Representative immunohistochemical staining of the skin using an anti-YAP antibody.

Article Snippet: Rabbit anti -GAPDH antibodies were obtained from Sigma-Aldrich (St. Louis, MO, USA), and rabbit anti -ABCB1, anti -SCD1, anti -NEDD4, anti -Ki67, anti -Loricrin, and anti-YAP antibodies were purchased from ProteinTech (Proteintech, Inc., USA).

Techniques: Immunohistochemical staining, Staining, Comparison, Gene Expression, Expressing

Journal: Materials Today Bio

Article Title: Axolotl-derived decellularized skin ECM as a pro-regenerative scaffold for attenuating fibrotic wound healing

doi: 10.1016/j.mtbio.2025.102443

Figure Lengend Snippet: Improved wound healing by A-dECM through restoration of epithelial thickness, skin architecture, and collagen deposition in thermal injury tissues. (A) MT staining of wound tissues captured at 20× magnification. Left: full field view; Right: cropped region from the same image. Both panels share the same scale bar = 100 μm, illustrating collagen deposition and dermal organization. A-dECM-treated tissues exhibited collagen architecture resembling that of normal skin, while M-dECM-treated tissues displayed irregular collagen patterns. (B) Epidermal thickness measurement in wound tissue. (C) Ratio of wound skin thickness relative to adjacent normal tissue. (D) Quantification of collagen density from MT-stained wound sections. (E) Immunohistochemical staining of the epidermal layer at the wound site on day 28 post-injury, showing expression of epithelial differentiation markers K5, K10, and Loricrin. Scale bar = 100 μm. (F) Quantification of layer-specific signal thickness for K5, K10, and Loricrin, expressed as percentage of total epidermal thickness at the wound center (PBS, M-dECM, A-dECM) and in normal skin. Data are presented as mean ± S.D. (n = 6 biologically independent animals per group). Image analysis was performed on 5 randomly selected fields per tissue section. ∗ denote p < 0.05, ∗∗ denotes p < 0.01, ∗∗∗∗ denotes p < 0.0001. Comparisons without statistical annotations were not statistically significant. Statistical analysis was performed using one-way ANOVA followed by post hoc multiple comparisons.

Article Snippet: Sections were then incubated overnight at 4 °C with primary antibodies against CD68 (Abcam, ab125212), iNOS (Thermo Fisher Scientific, PA1-036), CD163 (Abcam, ab182422), K5 (Abcam, ab52635), K10 (Abcam, ab76318), and Loricrin (Elabscience, Wuhan, China; E-AB-92323).

Techniques: Staining, Immunohistochemical staining, Expressing